Biochemical Techniques

课程简介

/ Indicative Syllabus INDICATIVE CONTENT • Principles & practices of chromatography – permeation, ion- exchange, affinity chromatography; gas chromatography; HPLC; separation of biomolecules 6 hours • Electrophoresis: principles and general techniques; special techniques-high voltage discontinuous, SDS-polyacrylamide, gradient, isoelectric focusing, two-dimensional electrophoresis and western blotting 6 hours • Fluorescence pri ncipl e and its applications; flow cytometry 8 hours • DNA microarray 2 hours • DNA sequencing and fingerprinting 2 hours • Luminescence and chemiluminescence; luciferase, green fluorescent protein 2 hours • Real-time PCR 2 hours • Sample preparation: cell disruption, buffer, dialysis, concentration methods, measurement of proteins and nucleic acids 2 hours • Centrifugation and subcellular fractionation 2 hours -- 1 of 3 --

目标

Through lectures, tutorials and practicals, this subject is intended to provide students with an understanding of the fundamental principles of instruments and techniques commonly used in biochemistry and molecular biology.

先修要求

Biochemistry

Teaching Pattern

Methodology Lectures will be used to introduce the basic principles. Emphasis is put on the application of these techniques, rather than memorizing the detailed principles. Exercises and case studies in tutorials are used to teach students how to analyze experimental data and design experiments to solve some basic biochemical problem. Practicals will be used to allow students to have a hands-on experience in purifying and analyzing proteins. Lab reports are used to teach proper write up and communication skills.