Biochemical Techniques Laboratory

课程简介

/ Indicative Syllabus • Principles & practices of chromatography – permeation, ion exchange, affinity chromatography; gas chromatography; HPLC; separation of biomolecules • Electrophoresis: principles and general techniques; special techniques-high voltage discontinuous, SDS-polyacrylamide, gradient, isoelectric focusing, two-dimensional electrophoresis and western blotting • Fluorescence principle and its applications; flow cytometry • DNA microarray • DNA sequencing and fingerprinting • Luminescence and chemiluminescence; luciferase, green fluorescent protein • Real-time PCR -- 1 of 2 -- 2 • Sample preparation: cell disruption, buffer, dialysis, concentration methods, measurement of proteins and nucleic acids • Centrifugation and subcellular fractionation

目标

This subject is intended to apply fundamental principles of instruments and techniques commonly used in biochemistry and molecular biology through experimentation.

先修要求

/ Co-requisite/ Exclusion General Laboratory Techniques and Safety, Biochemistry

Teaching Pattern

Methodology Practicals will be used to allow students to have a hands-on experience in purifying and analyzing proteins. Lab reports are used to teach proper write up and communication skills.