Chromatographic Analysis

课程简介

/ Indicative Syllabus General Principles and Basic Techniques of Separation Filtration; Crystallization; Distillation; Extraction; Paper Chromatography; Thin-layer Chromatography; Column Chromatography. High-Performance Liquid Chromatography Basic instrumentation; silica gel, bonded phase normal and reverse-phase packings; UV, fluorescence and MS detectors; Ion-exchange, size- exclusion, and affinity chromatography; new liquid chromatographic techniques. Gas Chromatography Basic instrumentation; types of columns; choice of stationary phases; properties of thermal-conductivity, flame ionization and electron capture detectors; temperature-programming; qualitative and quantitative analytical methodology. Fundamentals of Chromatography The chromatographic process; capacity factor and retention times; column efficiency and resolution; general chromatographic theory. Sample Preparation for Chromatographic Analysis Homogenization; extraction; cleanup.

目标

This module aims to educate students to understand the principles and applications of various chromatographic techniques.

先修要求

Chemical Principles for Testing and Analysis

Teaching Pattern

Methodology Lecture: basic principles will be introduced and discussed. Examples will be used to illustrate the applications of various techniques. Tutorials: a set of tutorial problems will be given to allow students to apply the knowledge acquired from the lecture. Students are encouraged -- 1 of 2 -- to solve the problems before seeking assistance. These will help students consolidate what they have learned and develop a deeper understanding of the subject.