Advanced Molecular Biology

课程简介

/ Indicative Syllabus DNA repair - DNA damage response - Detection and repair of DNA damages - Regulatory elements during DNA damage response - Effect of DNA damage on DNA replication - DNA repair systems: nucleotide excision repair system, non-homologous end joining and homologous recombination repair systems. Gene mapping in eukaryotes: - RFLPs and SNPs (single nucleotide polymorphism), and GWAS (genome- wide association study) - parametric and non-parametric methods - linkage analysis Non-Mendelian Inheritance - Extranuclear inheritance (mitochondrial genome) - genomic imprinting - Mosaicism Advanced Gene Regulation - Epigenetics and Chromatin remodeling - Post-translational modification: acetylation, methylation, phosphorylation and sumoylation - Transcriptional regulation - siRNA and miRNA (micro RNA) - Protein turnover: the degradation process, the proteasome, ubiquitination and targeting -- 1 of 2 --

目标

In this subject, students will understand the interactions between the various systems of a cell, including the interactions between DNA, RNA and protein, as well as learning how these interactions are regulated.

先修要求

Cell Biology, Molecular Biology

Teaching Pattern

Methodology The basic concepts of these molecular processes will be explained in lectures with the inclusion of interactive animations to aid the student to understand the more complex molecular events. In tutorials, students will have discussion sessions to enhance their learning while probing questions and exercises will be used to gauge the learning outcomes of the students. In laboratory sessions, students will have the chance to isolate total RNA from tissue samples and check its integrity. Students should also reinforce their learning through self-learning from the textbook and suggested review and original journal articles.